Notice of retraction
Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Portable Electrochemical Immunochromatography Platform for Point-of-care Testing and Comparison with Enzyme-linked Immunosorbent Assay

Wataru Iwasaki, Nobutomo Morita, and Masaya Miyazaki

(Received April 2, 2020; Accepted June 17, 2020)

Keywords: electrochemical measurement, immunochromatography, paper-based analytical device, point-of-care testing

We conducted electrochemical immunochromatography using a developed electrochemical immunochromatography platform. We used immunoglobulin G (IgG) as a representative protein. We also conducted enzyme-linked immunosorbent assay (ELISA) as a control test. We used the same reagents except for the substrate and compared their limits of detection (LODs) and detection ranges. We succeeded in measuring IgG concentrations ranging from 30 ng/ml to 10 µg/ml and obtained 23.00 ng/ml as the LOD for electrochemical immunochromatography. The detection signal was not saturated even at 10 µg/ml, so it seems that higher concentrations of IgG can also be measured. The LOD was approximately 80-fold higher than that of ELISA (0.30 ng/ml); however, the detection range was one order of magnitude higher than that of ELISA. While electrochemical immunochromatography is less sensitive than ELISA, we demonstrated sufficient sensitivity for its use in a point-of-care testing (POCT) device. Furthermore, this information will be very helpful when introducing an assay system already established in ELISA to POCT.

Corresponding author: Wataru Iwasaki




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