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Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials, Volume 28, Number 6 (2016)
Copyright(C) MYU K.K.
pp. 593-599
S&M1213 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2016.1277
Published: June 22, 2016

Micro-Polymerase Chain Reaction Chip Reaction Chamber Temperature Measurement [PDF]

Chan-Young Park, Seung-Cheol Lee, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim

(Received March 1, 2015; Accepted January 14, 2016)

Keywords: microfluidic channel, internal temperature, polymerase chain reaction, NTC-thermistor, thermocouple

In this paper, we discuss the correlation between the reagent temperature and the thermistor sensor outside the chamber. The polymerase chain reaction (PCR) chip employed is constructed on a printed circuit board (PCB) with a heating pattern and a thermistor at the opposite side of the chamber. Since the reaction chamber is made with various films and tapes, a thermocouple could be embedded easily to measure the reagent temperature within the chamber. The parameters for chip control, such as the maximum heating power and the temperature compensation coefficient, were determined using the measured temperatures. The results show that the parameters determined enabled the exact control of the reagent temperature during the polymerase chain reaction.

Corresponding author: Jong-Dae Kim


Cite this article
Chan-Young Park, Seung-Cheol Lee, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim, Micro-Polymerase Chain Reaction Chip Reaction Chamber Temperature Measurement, Sens. Mater., Vol. 28, No. 6, 2016, p. 593-599.



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