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Vol. 34, No. 8(3), S&M3042

Notice of retraction
Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Sensors and Materials, Volume 26, Number 10 (2014)
Copyright(C) MYU K.K.
pp. 737-744
S&M1037 Research Paper
https://doi.org/10.18494/SAM.2014.1026
Published: December 22, 2014

Signal Enhancement of Indirect-Competitive Metallothionein Immunosensor by Horseradish-Peroxidase-Induced Precipitation of Diaminobenzidine Derivatives [PDF]

Namsoo Kim

(Received February 17, 2014; Accepted September 12, 2014)

Keywords: signal enhancement, indirect-competitive metallothionein sensor, horseradish peroxidase (HRP)-induced precipitation, diaminobenzidine derivatives

An indirect-competitive quartz crystal microbalance immunosensor is well acknowledged as a tool for biological detection. As a method of improving sensitivity, horseradish-peroxidase-induced precipitation of 3,3’-diaminobenzidine (DAB) derivatives to the sensor surface, which was confirmed by field emission-scanning electron microscopy imaging, was conducted using metallothionein (MT) as a model analyte. The sensor signal was enhanced up to 5.3-fold when the DAB g/ml. Linearity in response signal was found in the MT range of 0.05–1 ng/ml, with the limit of detection of 50 pg/ml. The present biosensor can serve as a highly sensitive detection tool for biomarkers of physiological importance.

Corresponding author: Namsoo Kim


Cite this article
Namsoo Kim, Signal Enhancement of Indirect-Competitive Metallothionein Immunosensor by Horseradish-Peroxidase-Induced Precipitation of Diaminobenzidine Derivatives, Sens. Mater., Vol. 26, No. 10, 2014, p. 737-744.



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